Authentic Biochemistry

Dr Daniel J. Guerra

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Nov 12, 2020 · 29 min
Dr. Guerra explains the ID2 HLH pseudo transcriptional corridor in leukemia and aging. 11 November 2020
1.Id proteins are helix-loop-helix (HLH) proteins lacking the basic amino acid domain necessary to bind DNA.and therefore ID functions in a dominant negative manner by sequestering ubiquitously expressed or cell- restricted basic HLH transcription factors: this results in effective blockade of transcription since ID causes a failure of dimerized basic HLH proteins to bind DNA. 2. Id proteins regulate transcription factors that are involved in developmental processes such as myogenesis, neurogenesis, bone morphogenesis, lymphopoiesis hematopoiesis, and myeloid differentiation 3. Id2−/− mice lack lymph nodes and Peyer's patches*, *Peyer's patches are discrete masses of lymphatic tissue found throughout the ileum of the mammalian small intestine Oncogene volume 22, pages1–9(2003) PNAS January 15, 2019 116 (3) 890-899 SUBSCRIBE and tell a colleague about Authentic Biochemistry And please Donate to my podcast!
Nov 10, 2020 · 29 min
Multiple Transcription Factor functional integration from immune cell lineages coordinate gene expression that maintains responses to intestinal infection during aging
Since epithelial clock gene and lymphocyte transcription factor Nfil3 expression is elevated in recombination deficient mice the potential for increased bacterial populations lining the gut may induce an expansion of class 3 innate-like T lymphocytes (ILC3) in the small intestine. thus allowing for circadian clock activity maintenance and effective immune clearance of potential pathogens. This observation may provide a potential pathobiochemical link between obesity and the increasing pathophysiology associated with human aging. Dr. Daniel J. Guerra Authentic Biochemistry Publication 10 November 2020 PLEASE SUBSCRIBE and give a 5 star review for my podcast and DONATE!!!
Nov 10, 2020 · 29 min
Transcription Factor NFil3 tracks the circadian clock to microbiome linked immune cascade in the gut. Dr. Daniel J. Guerra 09 Nov. 2020
NFIL3 is a critical transcription factor immunologically linking the microbiome to the circadian clock and lipid metabolism where the Innate Lymphoid Cell subtype 3 and dendritic cells may stipulate the intestinal microfloral associated epithelial temporal chronicity. Subscribe to my Podcast and donate! Published by Dr Daniel J Guerra, Authentic Biochemistry 09 November 2020 Reference: Science. 2017 Sep 1; 357(6354): 912–916.
Nov 2, 2020 · 29 min
Dr Guerra illustrates that NFIL3 represses genes by recruiting Sirtuin 2 and G9a histone methyltransferase eo ipso regulating diverse biological processes,
Nuclear factor interleukin 3 (NFIL3, also known as E4-binding protein 4, E4BP4) is a repressor of numerous genes. NFIL3 contains a basic leucine zipper domain, comprising amino acids 73–146, among 462 residues; the N-terminal part of this domain directly binds to DNA, while the C-terminal region is responsible for homo- or heterodimerization of the protein. Amino acids 299–363 comprise a transcriptional repression domain where the N-terminal part of this domain directly binds to DNA, while the C-terminal region is responsible for homo- or heterodimerization of the protein and amino acids 299–363 comprise a transcriptional repression domain. Please SUBSCRIBE and help out with $ donations!
Oct 29, 2020 · 29 min
The Circadian clock and the Acquired Immune Response coordinate at the event of transcription factor mediated aging phenotype. Dr Daniel J. Guerra
Dr. Guerra hybridizes multiple strands of published biochemical research to obtain an event ontology for potentiating factors leading to the aging human pathophysio9logical phenotype. In this lecture I include the following detail: 1. Nuclear factor interleukin 3 (NFIL3, also known as E4-binding protein 4, E4BP4) is a repressor of numerous genes. where an N-terminal domain directly binds to its response element, while the C-terminal region is responsible for homo- or heterodimerization to induce a temporally synchronized geometric configuration obtaining as a transcriptional repression domain 2. NFIL3 represses genes by recruiting histone deacetylase 2 and G9a histone methyltransferase in diverse physiological networks including the circadian clock, acquired immune response, cell fate, and intermediary metabolism. Papers to read: Experimental & Molecular Medicine volume 51, Article number: 80 (2019) Journal of Molecular Endocrinology (2019) 63, R93–R102 SUPPORT AUTHENTIC BIOCHEMISTRY! Subscribe and support with cash donatives to keep Dr Guerra and his family in winter boots and scarves for the season ahead.
Oct 29, 2020 · 29 min
Animals have adapted behavioral responses corresponding with light and temperature events to respond to and anticipate basic bioenergetics due of heterotrophy. Authentic Biochemistry . 28/10/2020
Since space and time are never the same twice and indeed are relatively uncertain, not even the day/night chronicity is a true cycle. It is a pattern or a familiar sequence of events and nothing more. No two moments are identical and so our internal circadian clock tracks these patterns but the means by which this detection operates at the cellular and molecular level, as well as the event itself involving the revolving celestial bodies in constant flux so in time, the pattern becomes less uniform while the ideal presents as fixed and familiar so to be displayed intentionally and subsequently neurologically as apparent classical experiential phenomena. Feedback loops compose the ‘molecular clock’ which is governed by a cascading transcription and translational regulatory mechanisms that are sufficient to maintain circadian rhythms. Journal of Molecular Endocrinology (2019) 63, R93–R102
Oct 25, 2020 · 29 min
While Melatonin binding to the MT1 and MT2 receptors may benefit aging via suppression of oncogenic gene expression and enhancement of the SCN sleep wake cycle, receptor loss occurs with senescence.
The pharmaceutical Remelteon may be a better MT1 MT2 agonist mimetic for decreasing melatonin synthesis in the aging pineal because it fails to bind MT3 receptor which is an active quinone reductase 2 synthesizing superoxide system. Published 24 October 2020 by Authentic Biochemistry Podcast; Dr Daniel J. Guerra
Oct 22, 2020 · 28 min
L-TRP is precursor to MEL and NAD ;Mel regulates Sirtuins which Perform DSB repair; NAD metabolism after SIRT activity yields PAR for SSB repair. DJGPhD 21 Oct 2020.
The artificial enhancement of brain melatonin levels were hoped to suppress the progress of Alzheimer's Disease in humans and for correcting the circadian and sleep-wake disturbances associated with advanced aging.. However, given the complex association of melatonin on NAD+ tonicity and this relationship playing a role in DNA Damage Repair Responses (both SSB and DSB) . melatonin or a surrogate mimetic receptor agonist may have undesirable effects on neurodegeneration and oncogenesis in the CNS and the periphery. Dr Guerra opens this complex biochemical terrain in this and the previous 21 October Authentic Biochemistry podcast lectures. Please donate to AB! I need you ! •
Oct 21, 2020 · 30 min
The Association of NAD+, Melatonin, &Sirtuins via DNA Damage Repair and Epigentic Chromatin Remodeling with Immunosenescence is evident
This episode lays the foundation for a common precursor-the essential amino acid L-tryptophan, of both melatonin and NAD+ and the PARP mediated DNA repair pathways linked to tumorigenesis on one hand and in DNA Damage repair-linked cessation of apoptosis on the other, in the aging human Central Nervous System. Cells. 2019 Sep; 8(9): 1047. Int J Alzheimers Dis. 2011: 741974.
Oct 20, 2020 · 29 min
DDR and the roles of Sirtuins and the NAD metabolic paradigm in aging. Published by Authentic Biochemistry Dr. Daniel J. Guerra 19 Oct 2020
The pineal endocrine hormone melatonin proximally decreasesIL-1β-induced MMP production by inhibiting Sirt1-dependent NAMPT and NFAT5 signaling in chondrocytes; since autoinflammatory Osteoarthritis (OA) is a degenerative joint (cartilage degradation) disease linked to human aging and Interleukin-1β contributes to OA pathogenesis by enhancing oxidative stress and inflammation the role(s) of sirtuin and NAD+ metabolism is associated with aging morbidity while also being described in Double-Stranded Break (DBS) DNA Damage Repair (DDR), we have isolated and identified with distinction, pathobiochemical components of the human aging event ontology. Oncotarget. 2017 Aug 22; 8(34): 55967–55983. PLOS One .November 25, 2014https://doi.org/10.1371/journal.pone.0113939 Int J Mol Sci. 2019 Mar; 20(5): 1223. eLife 2020;9:e55828 DOI: 10.7554/eLife.55828
Oct 15, 2020 · 29 min
Dr Guerra presents :"Deacetylase Agency in NAD+ and ADP Ribose Metabolism as the Equipoise for Cell Fate In Aging and Disease" 14 Oct.2020
Dr. Guerra offers a deep investigation into the roles of sirtuins in homeostasis , aging and disease as linked to NAD+ metabolism via ecto enzymatic CD38 and intracellular forms of this NAD+ metabolizing enzyme that is associated with T lymphocyte bioenergetics, activation and regulation as embedded within the inflammatory response, disease and aging. Published in Authentic Biochemistry Podcast series on Aging and the immune system. 14 October 2020 Papers mentioned in today's lecture : Hogan, et al. Front. Immunol., 31 May 2019 | Cells 2020, 9(1), 228 Camacho-Pereira et al., 2016, Cell Metabolism 23, 1127–1139 Sci. Signal. 10, eaal3024 (2017) 17 October 2017 November 2011 Pharmacological reviews 64(1):166-87
Oct 12, 2020 · 29 min
Dr Daniel J. Guerra of Authentic Biochemistry lectures on Senescence in Human Aging: The Sirtuin Intrusion
Sirtuins are protein deacetylases with multiple isoforms active in the nucleus, cytosol and mitochondria all serving unique functions and requiring NAD+. These sirtuins function to condense chromatin and yet protein abundance has less to do with potency than does the availability of NAD+ which can be synthesized from multiple routes. Furthermore, SIRT1 has been associated with antagonizing age-related decreases in the amplitudes of the SCN output obtaining age-linked differentiating phases of increases and decreases observed within a circadian cycle.. References: Int J Mol Sci. 2019 Mar; 20(5): 1223. PLOS One November 25, 2014 https://doi.org/10.1371/journal.pone.0113939 Circ Res. 2018 Sep 14; 123(7): 868–885
Oct 8, 2020 · 29 min
Dr. Guerra obtains a dialectical analysis of the pineal hormone melatonin in deacetylase-mediated Respiratory disease and aging
It is proposed that the endocrine hormone melatonin may regulate after its synthesis and secretion from the pineal gland, the deacetylase acivity of Sirtuin1 to diminish the SASP repertoire of aging and may be linked to common morbidity of pathophysiology linked to COPD and generic Respiratory Distress Syndrome (RDS)presentation. Papers of Record in this episode Int J Mol Sci. 2019 Mar; 20(5): 1223. Int. J. Mol. Sci. 2014, 15, 16848-16884 International Immunopharmacology Volume 62, September 2018, Pages 23-28
Oct 8, 2020 · 29 min
Dr. Daniel Guerra presents Immuno Diaeventontological Framing of the Aging Human IV: SASP to cGAS via Melatonin
Dr. Guerra temporally organizes the lecture series toward the dialectical event by mindfully schematizing the SASP , the triad of DNA Damage Repair (DDR)responses, transcriptionally activated pro-inflammatory cytokine mediated inflammation, and the pineal-endocrine hormone melatonin circadian clock manifestations in the biochemistry and physiology of the aging human. Please SUBSCRIBE! and when you can PLEASE DONATE to your favorite authentic biochemist so he can continue to provide with august research science interrogation and analysis.
Oct 4, 2020 · 29 min
Dr. Guerra finds that the tumor-promoting and suppressing biochemical phenotypes of senescence are phenomenologically contrarion and thus physiologically compatible
Lipids, proteins carbohydrates and nucleotides-may function within an inflammatory and secretory modality. Thus, the SASP-Senescence-Associated Secretory Phenotype might deliver apoptosis, necroptosis, autophagy, oncogenesis or an extended senescence sequalae. An Authentic Biochemistry audio lecture podcast by Dr. Daniel J. Guerra. 04 October 2020. Please SUBSCRIBE , check out my video lectures ( https://www.youtube.com/channel/UCcEoA2N9WSlNvDk1BtuznfQ?view_as=subscriber) and donate! https://podbay.fm/p/authentic-biochemistry Grazie! Dr. Guerra Nature Reviews | Cancer Reviews 19 | AUGUST 2019 :pp. 439 Nature Communications Vol. 9, Article number: 1228 (2018) doi:10.1038/s41467-018-03566-5 Front. Genet., 12 March 2015 https://doi.org/10.3389/fgene.2015.00094
Sep 27, 2020 · 22 min
Is cancer. or survival-achieving chemotherapy to treat early-onset tumorigenesis, linked to late in life senescence and human aging? The CD8+ T lymphocyte link.
A take-home message from this research may be that the immunosenescent phenotype in the elderly, while associated with an increased rate of infections and a diminished effect of vaccines, and increased cancer susceptibility may be promoted by cancer and /or chemotherapy to cure the cancer. Both cancer and chemotherapy contribute to immune compromise and potential for gene mutations and epigenetic phenomena. Please subscribe to Authentic Biochemistry podcast and donate to its continued longevity!
Sep 22, 2020 · 30 min
Dr Guerra suggests targeting senescence as a means to alter the rate of aging is contrarion to healthy outcome because of derepression of tumorigenesis.
The human body is tempered by cellular fate paradigms that include senescence vs. proliferation during aging. A corruption in the senescnce associated secretory phenotype may be a proximal tareget to reduce aging morbidity but does it not open the door to oncogenesis and the potential for autoimmune disease? Comput Struct Biotechnol J. 2019; 17: 1151–1161 Nat Med. 2017 Jun; 23(6): 775–781 Support your biochemistry podcast! Be "Authentic" and send donations now! Dr Guerra thanks you !
Sep 20, 2020 · 29 min
Senescence and Aging Relative to T lymphocytes may be linked to chemotherapy of testicular cancer and underlying cell cycle-CD transcriptional mutations.
Dr Guerra continues his associative pathobiochemical dialectic concerning T lymphocytte differentaition and senescence The Th and Tc populations following uptake and processing of an antigen by an APC such as a dendritic cell is presented either to the CD8 population in the context of MHC-I or to the CD4 subpopulation in the context of MHC-II generates a cascading set of cellular lymphoproliferative and differentiative steps initiated under the inductive influence of cytokines that ultimately determine effector functions. Papers examined in this episode: Nat Med. 2017 Jun; 23(6): 775–781 Cancer Genet Cytogenet. 1999 Mar;109(2):108-13. BMC Cancer volume 20, Article number: 882 (2020)
Sep 18, 2020 · 28 min
Thymic involution-associated naive cd4+/cd8+ Tcell differentiation as observed in care-giver neuropsychological conditioning toward non-chronological immunosenescence.
Dr Guerra swings hard at Treg and naive CD4+/CD8+ lymphocytes in immunosenescence and the potential for infection with aging while accumulating T memory cell lineages confined to antigen./pathogen exposure including vaccine-associated immunizations. Newe paper disussed today: .Brain Behav Immun 2018 Oct; 73: 546–549.doi: 10.1016/j.bbi.2018.06.019. Epub 2018 Jun 22. PLeasse donate to Authentic Biochemistry! I need your financial support in these trying times.
Sep 17, 2020 · 28 min
The teleology of Thymus mediated T regulatory cell clonal elimination and expansion via membrane associated receptor ligand interactions with innate and lymphocyte linneages. Dr Daniel J. GUERRA
1. Age -associated Immunosenescence is sometimes treated as synonymous with immune insufficiency, resulting in enhanced random infections and reduced vaccine immunity, and tumor surveillance 2. Self-reactive immune responses are elevated in the elderly, which is a result of inflammaging, a chronic, low-grade, systemic pro-inflammatory phenotype in the absence of acute infection 3. Immunosenescence and inflammaging are pathophysiological, and can be the immune poise for hyperinflammation and autoimmune disease in the elderly, leading to severe morbidity and increased mortality. Please subscribe and support our podcast with funding!